After you create an Autoplay, the following status will be displayed: "Autoplay enabled. Order pending". This means an order for the upcoming drawing will be placed within 15 minutes. If you choose a jackpot threshold, then the order will only be placed if the threshold is met. 

Each subsequent order will be placed when the new jackpot is known if that jackpot meets the threshold you specified. Because states do not release the new jackpot at the exact same time after every drawing, there will be some variation as to when your next Autoplay order executes.
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